Functional tissues generated under in vitro conditions are urgently needed in biomedical research. However, the engineering of tissues is rather difficult, since their development is influenced by numerous parameters. In consequence, a versatile culture system was developed to respond the unmet needs.Optimal adhesion for cells in this system is reached by the selection of individual biomaterial...

Recent reports on work safety in container shipping operations highlight high frequencies of human failures. In this study, we empirically examine the effects of seafarers' perceptions of national culture on the occurrence of human failures affecting work safety in shipping operations. We develop a model adopting Hofstede's national culture construct, which comprises five dimensions, namely pow...

Nowadays, the most rapid method for producing healthy and disease-free Lisiantus is micropropagation. With respect to high economic value of this plant which is regarded among the 10 top cutting flowers in the world, this research was carried out to suggest a suitable protocol for its in vitro propagation, using nodal sections as an explant. To carry out this object, the effects of the pH (5.5,...

To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed. First an individual biomaterial is selected and then mounted in a MINUSHEET(®) tissue carrier. After sterilization the assembly is transferred by fine forceps to a 24 well culture plate for seeding cells or...

Immediate culture or refrigeration of urine is recommended, but not always practical. Therefore, we evaluated the Becton-Dickinson Urine Culture Kit containing a boric acid-glycerol-sodium formate preservative in a study of 1,000 clinical urine specimens. Each specimen was cultured a total of four times by the surface streak technique with a 0.001-ml calibrated loop. After an initial reference ...

As a multiple function tree species, Cyclocarya paliurus (Batal) Iljinskaja is mainly planted and managed for timber production and medical use. To improve the seed use efficiency and outplanting performance of C. paliurus, the effects of container types and growth medium on the seedling growth and root morphology of C. paliurus were investigated by using a completely randomized block experimen...

BACKGROUND We developed a new disposable anaerobic culture system, namely, the Quick anaero-system, for easy culturing of obligate anaerobes. METHODS Our system consists of 3 components: 1) new disposable anaerobic gas pack, 2) disposable culture-envelope and sealer, and 3) reusable stainless plate rack with mesh containing 10 g of palladium catalyst pellets. To evaluate the efficiency of our...

Dissociated hippocampal neuron culture has long been the model system of choice for many neuroscientists. The ability to culture dissociated hippocampal neurons from genetically modified mice provides an invaluable tool for studying many neuronal processes. In this study, we established a novel method to culture dissociated hippocampal neurons from embryonic and neonatal mice. Dissociated neuro...